NEGATIVE STAINING OF FRESH-WATER BACTERIONEUSTON SAMPLED DIRECTLY WITH ELECTRON-MICROSCOPE SPECIMEN SUPPORT GRIDS

被引：5

作者：

FUERST, JA ^{[1
]}

MCGREGOR, A ^{[1
]}

DICKSON, MR ^{[1
]}

机构：

[1] UNIV NEW S WALES, BIOMED ELECTRON MICROSCOPE UNIT, KENSINGTON, NSW 2033, AUSTRALIA

来源：

MICROBIAL ECOLOGY | 1987年 / 13卷 / 03期

关键词：

D O I：

10.1007/BF02024999

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

A technique for observation of surface microlayer bacteria (bacterioneuston) is described, utilizing direct sampling of the air-water interface with carbon-stabilized electron microscope specimen support grids, followed by negative staining and transmission electron microscopy. The method resulted in excellent preservation of forms of microcolonial association, regular surface arrays, surface appendages, and prosthecae in the bacterioneuston of a freshwater pond.

引用

页码：219 / 228

页数：10

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