4 NEW DERIVATIVES OF THE BROAD-HOST-RANGE CLONING VECTOR PBBR1MCS, CARRYING DIFFERENT ANTIBIOTIC-RESISTANCE CASSETTES

被引：2897
作者：
KOVACH, ME
ELZER, PH
HILL, DS
ROBERTSON, GT
FARRIS, MA
ROOP, RM
PETERSON, KM
机构：
[1] LOUISIANA STATE UNIV,MED CTR,DEPT MICROBIOL & IMMUNOL,SHREVEPORT,LA 71130
[2] CIBA AGR BIOTECHNOL,RES TRIANGLE PK,NC 27709
来源：
GENE | 1995年 / 166卷 / 01期
关键词：
PLASMID; MOBILIZABLE VECTOR; MULTIPLE CLONING SITE; LACZ-ALPHA COMPLEMENTATION;
D O I：
10.1016/0378-1119(95)00584-1
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Four new antibiotic-resistant derivatives of the broad-host-range (bhr) cloning vector pBBR1MCS have been constructed, These new plasmids have several advantages over many of the currently available bhr vectors in that: (i) they are relatively small (<5.3 kb), (ii) they possess an extended multiple cloning site (MCS), (iii) they allow direct selection of recombinant plasmid molecules in Escherichia coli via disruption of the LacZ alpha peptide, (iv) they are mobilizable when the RK2 transfer functions are provided in trans and (v) they are compatible with IncP, IncQ and IncW group plasmids, as well as with ColE1- and P15a-based replicons.
引用
收藏
页码：175 / 176
页数：2
相关论文
共 9 条
[1]   ISOLATION AND MOLECULAR CHARACTERIZATION OF A NOVEL BROAD-HOST-RANGE PLASMID FROM BORDETELLA-BRONCHISEPTICA WITH SEQUENCE SIMILARITIES TO PLASMIDS FROM GRAM-POSITIVE ORGANISMS [J].
ANTOINE, R ;
LOCHT, C .
MOLECULAR MICROBIOLOGY, 1992, 6 (13) :1785-1799
[2]   CONSTRUCTION AND CHARACTERIZATION OF NEW CLONING VEHICLES .2. MULTIPURPOSE CLONING SYSTEM [J].
BOLIVAR, F ;
RODRIGUEZ, RL ;
GREENE, PJ ;
BETLACH, MC ;
HEYNEKER, HL ;
BOYER, HW ;
CROSA, JH ;
FALKOW, S .
GENE, 1977, 2 (02) :95-113
[3]   IN-VIVO AND IN-VITRO STABILITY OF THE BROAD-HOST-RANGE CLONING VECTOR PBBR1MCS IN 6 BRUCELLA SPECIES [J].
ELZER, PH ;
KOVACH, ME ;
PHILLIPS, RW ;
ROBERTSON, GT ;
PETERSON, KM ;
ROOF, RM .
PLASMID, 1995, 33 (01) :51-57
[4]   A SIMPLE PROCEDURE FOR TRANSFERRING GENES CLONED IN ESCHERICHIA-COLI VECTORS INTO OTHER GRAM-NEGATIVE BACTERIA - PHENOTYPIC ANALYSIS AND MAPPING OF TOL PLASMID GENE XYLK [J].
HARAYAMA, S ;
REKIK, M .
GENE, 1989, 78 (01) :19-27
[5]   IMPROVED BROAD-HOST-RANGE PLASMIDS FOR DNA CLONING IN GRAM-NEGATIVE BACTERIA [J].
KEEN, NT ;
TAMAKI, S ;
KOBAYASHI, D ;
TROLLINGER, D .
GENE, 1988, 70 (01) :191-197
[6]  
KOVACH ME, 1994, BIOTECHNIQUES, V16, P800
[7]   TNPHOA - A TRANSPOSON PROBE FOR PROTEIN EXPORT SIGNALS [J].
MANOIL, C ;
BECKWITH, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (23) :8129-8133
[8]   CONSTRUCTION AND USE OF A NEW BROAD-HOST-RANGE LACZ TRANSCRIPTIONAL FUSION VECTOR, PHRP309, FOR GRAM(-) BACTERIA [J].
PARALES, RE ;
HARWOOD, CS .
GENE, 1993, 133 (01) :23-30
[9]  
SCHMIDHAUSER TJ, 1988, VECTORS SURVEY MOL C, P287
1