4 NEW DERIVATIVES OF THE BROAD-HOST-RANGE CLONING VECTOR PBBR1MCS, CARRYING DIFFERENT ANTIBIOTIC-RESISTANCE CASSETTES

被引：2865

作者：

KOVACH, ME

ELZER, PH

HILL, DS

ROBERTSON, GT

FARRIS, MA

ROOP, RM

PETERSON, KM

机构：

[1] LOUISIANA STATE UNIV,MED CTR,DEPT MICROBIOL & IMMUNOL,SHREVEPORT,LA 71130

[2] CIBA AGR BIOTECHNOL,RES TRIANGLE PK,NC 27709

来源：

GENE | 1995年 / 166卷 / 01期

关键词：

PLASMID; MOBILIZABLE VECTOR; MULTIPLE CLONING SITE; LACZ-ALPHA COMPLEMENTATION;

D O I：

10.1016/0378-1119(95)00584-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Four new antibiotic-resistant derivatives of the broad-host-range (bhr) cloning vector pBBR1MCS have been constructed, These new plasmids have several advantages over many of the currently available bhr vectors in that: (i) they are relatively small (<5.3 kb), (ii) they possess an extended multiple cloning site (MCS), (iii) they allow direct selection of recombinant plasmid molecules in Escherichia coli via disruption of the LacZ alpha peptide, (iv) they are mobilizable when the RK2 transfer functions are provided in trans and (v) they are compatible with IncP, IncQ and IncW group plasmids, as well as with ColE1- and P15a-based replicons.

引用

页码：175 / 176

页数：2

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