ULTRASTRUCTURAL MESSENGER-RNA DETECTION - INSITU HYBRIDIZATION OF A BIOTIN-LABELED OLIGONUCLEOTIDE PRIOR TO EMBEDDING

被引：0
|
作者：
TREMBLEAU, A
机构：
来源：
PATHOLOGIE BIOLOGIE | 1993年 / 41卷 / 02期
关键词：
INSITU HYBRIDIZATION; ELECTRON MICROSCOPY; HYPOTHALAMUS; MESSENGER RNA; VASOPRESSIN;
D O I：
暂无
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
A technique for mRNA detection by ultrastructural in situ hybridization is reported. Vibratome sections are hybridized with a biotinylated oligonucleotide probe which is detected using an enhanced avidine-peroxidase technique. Sections are then osmicated and embedded in epoxy resin. This rapid, sensitive technique achieved ultrastructural detection of vasopressin mRNA in magnocellular neurons of rat hypothalamus.
引用
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页码：194 / 197
页数：4
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