PRODUCTION OF MONOCLONAL-ANTIBODIES TO PSEUDOMONAS-SYRINGAE PV PHASEOLICOLA AND XANTHOMONAS-CAMPESTRIS PV PHASEOLI

被引：8
|
作者：
WONG, WC
机构：
[1] Department of Primary Industries, Plant Pathology Branch, Indooroopilly, Queensland, 4068, Meiers Road
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1990年 / 10卷 / 06期
关键词：
D O I：
10.1111/j.1472-765X.1990.tb00117.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The production of monoclonal antibodies (MAbs) to ethylenediamine tetraacetic acid (sodium salt) soluble antigens of Pseudomonas syringae pv. phaseolicola and X anthomonas campestris pv. phaseoli (fuscans strain) is described. MAbs A6‐1 and A6‐2 produced to Ps. syringae pv. phaseolicola are pathovar specific. Although MAb XP2 produced to X. campestris pv. phaseoli recognized surface antigens of all strains of this pathovar (including fuscans strains) it cross‐reacted specifically with X. campestris pv. malvacearum; it did not react with any other known bacteria or unidentified epiphytes from navy bean seed or leaves. The isotype of both MAbs XP2 and A6‐1 is IgG3 whereas that of MAb A6‐2 is IgG2a The reactive antigens are thermostable, but their chemical nature has not been determined. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
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页码：241 / 244
页数：4
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