STABILIZATION OF HUMAN RHINOVIRUS SEROTYPE-2 AGAINST PH-INDUCED CONFORMATIONAL CHANGE BY ANTIVIRAL COMPOUNDS

被引：46

作者：

GRUENBERGER, M

PEVEAR, D

DIANA, GD

KUECHLER, E

BLAAS, D

机构：

[1] UNIV VIENNA,INST BIOCHEM,WAHRINGERSTR 17,A-1090 VIENNA,AUSTRIA

[2] STERLING RES GRP,DEPT VIROL & ONCOPHARMACOL,RENSSELAER,NY 12144

[3] STGRLING RES GRP,DEPT MED CHEM,RENSSELAER,NY 12144

来源：

JOURNAL OF GENERAL VIROLOGY | 1991年 / 72卷

关键词：

D O I：

10.1099/0022-1317-72-2-431

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Four WIN compounds with anti-picornavirus activities were tested for their ability to stabilize human rhinovirus serotype 2 (HRV-2) against low pH-induced conformational changes in vitro, as determined by specific immunoprecipitation. These results were compared to the minimal inhibitory concentration (MIC) as measured in a plaque reduction assay. A direct relationship was observed between the concentration of the compound that prevented the low pH-induced conformational changes and the MIC, indicating that stabilization is an important element in the mode of action of these drug against HRV-2.

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页码：431 / 433

页数：3

相关论文

共 14 条

[1] 2 THRESHOLD VALUS OF LOW PH BLOCK ENDOCYTOSIS AT DIFFERENT STAGES [J].

DAVOUST, J ;

GRUENBERG, J ;

HOWELL, KE .

EMBO JOURNAL, 1987, 6 (12) :3601-3609

[2] ENTRY OF SEMLIKI FOREST VIRUS INTO BHK-21-CELLS [J].

HELENIUS, A ;

KARTENBECK, J ;

SIMONS, K ;

FRIES, E .

JOURNAL OF CELL BIOLOGY, 1980, 84 (02) :404-420

[3] CRYSTAL-STRUCTURE OF HUMAN RHINOVIRUS SEROTYPE-1A (HRV1A) [J].

KIM, S ;

SMITH, TJ ;

CHAPMAN, MS ;

ROSSMANN, MG ;

PEVEAR, DC ;

DUTKO, FJ ;

FELOCK, PJ ;

DIANA, GD ;

MCKINLAY, MA .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 210 (01) :91-111

[4] NATURALLY OCCURRING AND ARTIFICIALLY PRODUCED COMPONENTS OF 3 RHINOVIRUSES [J].

KORANT, BD ;

LONBERGH.K ;

STASNY, JT ;

NOBLE, J .

VIROLOGY, 1972, 48 (01) :71-&

[5] TRYPSIN SENSITIVITY OF SEVERAL HUMAN RHINOVIRUS SEROTYPES IN THEIR LOW PH-INDUCED CONFORMATION [J].

KOWALSKI, H ;

MAURERFOGY, I ;

VRIEND, G ;

CASARI, G ;

BEYER, A ;

BLAAS, D .

VIROLOGY, 1989, 171 (02) :611-614

[6] PHYSICAL AND METABOLIC REQUIREMENTS FOR EARLY INTERACTION OF POLIOVIRUS AND HUMAN RHINOVIRUS WITH HELA-CELLS [J].

LONBERGHOLM, K ;

WHITELEY, NM .

JOURNAL OF VIROLOGY, 1976, 19 (03) :857-870

[7] DIFFERENT PH REQUIREMENTS FOR ENTRY OF THE 2 PICORNAVIRUSES, HUMAN RHINOVIRUS-2 AND MURINE ENCEPHALOMYOCARDITIS VIRUS [J].

MADSHUS, IH ;

OLSNES, S ;

SANDVIG, K .

VIROLOGY, 1984, 139 (02) :346-357

[8] REQUIREMENTS FOR ENTRY OF POLIOVIRUS RNA INTO CELLS AT LOW PH [J].

MADSHUS, IH ;

OLSNES, S ;

SANDVIG, K .

EMBO JOURNAL, 1984, 3 (09) :1945-1950

[9] MECHANISM OF ENTRY OF HUMAN RHINOVIRUS-2 INTO HELA-CELLS [J].

NEUBAUER, C ;

FRASEL, L ;

KUECHLER, E ;

BLAAS, D .

VIROLOGY, 1987, 158 (01) :255-258

[10] CONFORMATIONAL CHANGE IN THE FLOOR OF THE HUMAN RHINOVIRUS CANYON BLOCKS ADSORPTION TO HELA-CELL RECEPTORS [J].

PEVEAR, DC ;

FANCHER, MJ ;

FELOCK, PJ ;

ROSSMANN, MG ;

MILLER, MS ;

DIANA, G ;

TREASURYWALA, AM ;

MCKINLAY, MA ;

DUTKO, FJ .

JOURNAL OF VIROLOGY, 1989, 63 (05) :2002-2007