ANALYSIS OF PEPTIDOGLYCAN PRECURSORS IN VANCOMYCIN-RESISTANT ENTEROCOCCUS-GALLINARUM BM4174

被引：103

作者：

REYNOLDS, PE ^{[1
]}

SNAITH, HA ^{[1
]}

MAGUIRE, AJ ^{[1
]}

DUTKAMALEN, S ^{[1
]}

COURVALIN, P ^{[1
]}

机构：

[1] INST PASTEUR,F-75724 PARIS 15,FRANCE

来源：

BIOCHEMICAL JOURNAL | 1994年 / 301卷

关键词：

D O I：

10.1042/bj3010005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vancomycin resistance in enterococci is an increasing clinical problem, and several phenotypes have been identified. We demonstrate here that the resistance mechanism in the constitutively vancomycin-resistant Enterococcus gallinarum BM4174 involves an altered pathway of peptidoglycan synthesis and hydrolysis of the normal precursors in the vancomycin-sensitive pathway. A ligase encoded by the vanC gene catalyses synthesis of D-Ala-D-Ser and substitutes this dipeptide for D-Ala-D-Ala in peptidoglycan precursors. It is presumed that this substitution lowers the affinity of vancomycin for its target site. Destruction of D-Ala-D-Ala (D,D-peptidase activity) and of UDP-MurNAc-L-Ala-D-isoGlu-L-Lys-D-Ala-D-ala by removal of the terminal D-Ala residue (D,D-carboxypeptidase activity) ensures that the normal vancomycin-sensitive pathway of peptidoglycan synthesis cannot function in the resistant strain.

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页码：5 / 8

页数：4

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