HIGH MOLECULAR-WEIGHT FORMS OF RELAXIN IN PREGNANT SOW OVARIES

被引：20

作者：

KWOK, SCM ^{[1
]}

CHAMLEY, WA ^{[1
]}

BRYANTGREENWOOD, GD ^{[1
]}

机构：

[1] UNIV HAWAII, JOHN A BURNS SCH MED, HONOLULU, HI 96822 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1978年 / 82卷 / 03期

关键词：

D O I：

10.1016/0006-291X(78)90882-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The existence of a prohormone for relaxin has been investigated by purification from extracts of pregnant sow ovaries. Radioimmunoassay of column fractions from large scale extraction of frozen pregnant sow ovaries detected two species of high molecular weight relaxin immunoactivity besides the 6,300 dalton relaxin. On further purification, these two species were resolved into three forms with apparent molecular weights of 19,000, 13,000 and 10,000 daltons respectively. Each was biologically active and could be converted by trypsin to the 6,300 dalton relaxin. They may represent intermediates of relaxin. © 1978.

引用

页码：997 / 1005

页数：9

共 27 条

[1]

AFELE S, UNPUBLISHED

[2] RELAXIN HAS CONFORMATIONAL HOMOLOGY WITH INSULIN [J].

BEDARKAR, S ;

TURNELL, WG ;

BLUNDELL, TL ;

SCHWABE, C .

NATURE, 1977, 270 (5636) :449-451

[3]

BELT WD, 1971, ENDOCRINOLOGY, V89, P1

[4] LABELING OF PROTEINS TO HIGH SPECIFIC RADIOACTIVITIES BY CONJUGATION TO A I-125-CONTAINING ACYLATING AGENT - APPLICATION TO RADIOIMMUNOASSAY [J].

BOLTON, AE ;

HUNTER, WM .

BIOCHEMICAL JOURNAL, 1973, 133 (03) :529-538

[5] SPECIFICITY OF A RADIOIMMUNOASSAY FOR RELAXIN [J].

BRYANT, GD ;

STELMASIAK, T .

ENDOCRINE RESEARCH COMMUNICATIONS, 1974, 1 (5-6) :415-433

[6]

CHAMLEY WA, ENDOCRINOLOGY

[7] PORCINE PROINSULIN - CHARACTERIZATION AND AMINO ACID SEQUENCE [J].

CHANCE, RE ;

ELLIS, RM ;

BROMER, WW .

SCIENCE, 1968, 161 (3837) :165-&

[8] HIGH MOLECULAR-WEIGHT FORMS OF ADRENOCORTICOTROPIC HORMONE IN MOUSE PITUITARY AND IN A MOUSE PITUITARY TUMOR-CELL LINE [J].

EIPPER, BA ;

MAINS, RE .

BIOCHEMISTRY, 1975, 14 (17) :3836-3844

[9]

FRIEDEN EH, 1977, P SOC EXP BIOL MED, V154, P407

[10]

Hisaw FL, 1926, P SOC EXP BIOL MED, V23, P661

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