A RADIOIMMUNOASSAY FOR HUMAN URINARY PREALBUMIN

被引：8

作者：

BEETHAM, R

DAWNAY, A

GHANY, C

DUBREY, S

MILES, J

机构：

[1] WESTMINSTER MED SCH & HOSP,DEPT IMMUNOL,LONDON SW1P 2AP,ENGLAND

[2] ST BARTHOLOMEWS HOSP,DEPT CHEM PATHOL,LONDON EC1A 7BE,ENGLAND

[3] CHARING CROSS & WESTMINSTER HOSP,SCH MED,DEPT CARDIOVASC MED,LONDON,ENGLAND

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1993年 / 30卷

关键词：

ASSAY VALIDATION; REFERENCE DATA; STABILITY ON STORAGE; PREALBUMIN CLEARANCE;

D O I：

10.1177/000456329303000405

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We describe a validated radioimmunoassay for prealbumin in urine. Using timed overnight urine samples, the normal reference range was less than 10-148 mug/L; or less than 1.8-9.6 mug/mmol creatinine, excretion in women being significantly greater than in men (P<0.05); or less than 7.3-114 ng/min with no significant difference in excretion rate between the sexes. Urines exhibited loss of immunoreactivity after storage at 20-degrees-C and thawing. No such loss occurred after storage for 4 weeks at 4-degrees-C or room temperature. The urinary excretion of prealbumin was highly correlated with that of albumin (r=0.85), and clearance relative to creatinine was 2 x 10(-6), the same order as that of albumin.

引用

页码：377 / 382

页数：6

共 12 条

[1]

BEETHAM R, 1985, CLIN CHEM, V31, P1364

[2]

BEETHAM R, 1982, ANN CLIN BIOCHEM, V29, P450

[3] COMPARISON OF RETINOL-BINDING PROTEIN AND BETA-MICROGLOBULIN DETERMINATION IN URINE FOR THE EARLY DETECTION OF TUBULAR PROTEINURIA [J].