HIGH-FREQUENCY TRANSFORMATION OF BACILLUS-SUBTILIS PROTOPLASTS BY PLASMID DNA

被引：1005

作者：

CHANG, S ^{[1
]}

COHEN, SN ^{[1
]}

机构：

[1] STANFORD UNIV,SCH MED,DEPT MED,STANFORD,CA 94305

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 168卷 / 01期

关键词：

D O I：

10.1007/BF00267940

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycolinduced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4x107 transformants per μg of supercoiled DNA. Plasmids constructed by in vitro ligation or endonuclease-generated fragments of linear plasmid DNA can also transform PEG-treated protoplasts, but at a lower frequency. © 1979 Springer-Verlag.

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页码：111 / 115

页数：5

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