EFFECTS OF SERUM-ISOLATED VS SYNTHETIC BILIRUBIN ALBUMIN COMPLEXES ON DYE-BINDING METHODS FOR ESTIMATING SERUM-ALBUMIN

被引：0

作者：

IHARA, H ^{[1
]}

NAKAMURA, H ^{[1
]}

AOKI, Y ^{[1
]}

AOKI, T ^{[1
]}

YOSHIDA, M ^{[1
]}

机构：

[1] TOHO UNIV,FAC SCI,DEPT BIOL,TOKYO 143,JAPAN

来源：

CLINICAL CHEMISTRY | 1991年 / 37卷 / 07期

关键词：

PROTEINS; DYE-BINDING COLORIMETRIC ASSAYS; ANALYTICAL ERROR; HYPOALBUMINEMIA;

D O I：

暂无

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Bush and Reed reported (Clin Chem 1987;33:821-3) that the reaction of albumin with bromcresol purple but not with bromcresol green underestimated the concentration of albumin in synthetically obtained bilirubin-albumin (Bd) by 29%. Their unproven assumption was that chemically synthesized Bd behaved in a manner indistinguishable from the natural Bd in icteric serum. Here we verify that Bd, whether synthetically obtained or isolated from serum, causes an underestimation of albumin in the bromcresol purple but not in the bromcresol green method. The molar ratio of Bd from either source to underestimated albumin approximates 1.0, suggesting that one molecule of Bd would react equivalently to a molecule of albumin in the bromcresol purple method. This underestimation might falsely suggest hypoalbuminemia in patients with increased serum Bd.

引用

页码：1269 / 1272

页数：4

共 16 条

[1]

BUSH V, 1987, CLIN CHEM, V33, P821

[2]

DOUMAS BT, 1985, CLIN CHEM, V31, P1779

[3]

DOUMAS BT, 1987, CLIN CHEM, V33, P769

[4] ALBUMIN STANDARDS AND MEASUREMENT OF SERUM ALBUMIN WITH BROMCRESOL GREEN [J].