REACTION OF RABBIT MUSCLE CREATINE-KINASE WITH DIETHYL PYROCARBONATE

被引:19
作者
CLARKE, DE [1 ]
PRICE, NC [1 ]
机构
[1] UNIV STIRLING,DEPT BIOCHEM,STIRLING FK9 4LA,SCOTLAND
关键词
D O I
10.1042/bj1810467
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The reaction of rabbit muscle creatine kinase with diethyl pyrocarbonate was studied. It was found that up to five of the sixteen histidine groups per enzyme subunit could be modified, and under the conditions employed, there was no evidence for formation of the disubstituted derivative of histidine. Evidence was obtained for small but significant amounts of modification of lysine and cysteine groups; tyrosine groups were not modified. Modification of the enzyme led to inactivation; this could be protected against by inclusion of substrates or, more effectively, by inclusion of the combination MgADP plus creatine plus nitrate, which is thought to produce a 'transition-stage-analogue' complex. Analysis of data on the rates of inactivation and the stoicheiometry of modification suggested that there was one essential histidine group per enzyme subunit, modification of which led to inactivation.
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页码:467 / 475
页数:9
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