GLUCOSYLTRANSFERASE GENE POLYMORPHISM AMONG STREPTOCOCCUS-MUTANS STRAINS

被引：31

作者：

CHIA, JS ^{[1
]}

HSU, TY ^{[1
]}

TENG, LJ ^{[1
]}

CHEN, JY ^{[1
]}

HAHN, LJ ^{[1
]}

YANG, CS ^{[1
]}

机构：

[1] NATL TAIWAN UNIV,COLL MED,SCH MED TECHNOL,TAIPEI,TAIWAN

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 05期

关键词：

D O I：

10.1128/IAI.59.5.1656-1660.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Genetic polymorphisms in genes coding for the glucosyltransferases were detected among Streptococcus mutans serotype c strains by Southern blot analysis with DNA probes located within the gtfB gene (H. Aoki, T. Shiroza, M. Hayakawa, S. Sato, and H. K. Kuramitsu, Infect. Immun. 53:587-594, 1986). Restriction endonucleases were used to examine genomic DNAs isolated from serotype a to h strains. The variations were readily detected among 33 strains of serotype c by EcoRI and PstI restriction enzyme digestions. Serotypes e and f, which are genetically similar to serotype c, also had comparable polymorphisms; however, serotypes a, b, d, g, and h did not hybridize to the same DNA probes in parallel experiments. Further analysis of enzymatic activities for glucan synthesis and sucrose-dependent adherence revealed no significant differences among the serotype c strains. Our results suggested that genetic polymorphisms existing in S. mutans serotype c strains may reflect a complexity in genes coding for the glucosyltransferases, which are produced ubiquitously in members of the S. mutans group.

引用

页码：1656 / 1660

页数：5

共 23 条

[1] CLONING OF A STREPTOCOCCUS-MUTANS GLUCOSYLTRANSFERASE GENE CODING FOR INSOLUBLE GLUCAN SYNTHESIS [J].

AOKI, H ;

SHIROZA, T ;

HAYAKAWA, M ;

SATO, S ;

KURAMITSU, HK .

INFECTION AND IMMUNITY, 1986, 53 (03) :587-594

[2] TIGHT GENETIC-LINKAGE OF A GLUCOSYLTRANSFERASE AND DEXTRANASE OF STREPTOCOCCUS-MUTANS GS-5 [J].

BURNE, RA ;

RUBINFELD, B ;

BOWEN, WH ;

YASBIN, RE .

JOURNAL OF DENTAL RESEARCH, 1986, 65 (12) :1392-1401

[3] GENTLE METHOD FOR LYSIS OF ORAL STREPTOCOCCI [J].

CHASSY, BM .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 68 (02) :603-608

[4]

CHIA JS, 1989, J FORMOS MED ASSOC, V88, P589

[5] GENETIC HETEROGENEITY IN STREPTOCOCCUS-MUTANS [J].

COYKENDA.AL .

JOURNAL OF BACTERIOLOGY, 1971, 106 (01) :192-&

[6] NUCLEOTIDE-SEQUENCE OF A GLUCOSYLTRANSFERASE GENE FROM STREPTOCOCCUS-SOBRINUS MFE28 [J].

FERRETTI, JJ ;

GILPIN, ML ;

RUSSELL, RRB .

JOURNAL OF BACTERIOLOGY, 1987, 169 (09) :4271-4278

[7] BIOLOGY, IMMUNOLOGY, AND CARIOGENICITY OF STREPTOCOCCUS-MUTANS [J].

HAMADA, S ;

SLADE, HD .

MICROBIOLOGICAL REVIEWS, 1980, 44 (02) :331-384

[8] ADHERENCE OF STREPTOCOCCUS-SANGUIS CLINICAL ISOLATES TO SMOOTH SURFACES AND INTERACTION OF THE ISOLATES WITH STREPTOCOCCUS-MUTANS GLUCOSYLTRANSFERASE [J].

HAMADA, S ;

TORII, M ;

KOTANI, S ;

TSUCHITANI, Y .

INFECTION AND IMMUNITY, 1981, 32 (01) :364-372

[9] EPIDEMIOLOGICAL SURVEY OF STREPTOCOCCUS-MUTANS AMONG JAPANESE CHILDREN - IDENTIFICATION AND SEROLOGICAL TYPING OF ISOLATED STRAINS [J].

HAMADA, S ;

MASUDA, N ;

OOSHIMA, T ;

SOBUE, S ;

KOTANI, S .

JAPANESE JOURNAL OF MICROBIOLOGY, 1976, 20 (01) :33-44

[10] ISOLATION AND CHARACTERIZATION OF THE STREPTOCOCCUS-MUTANS GTFC GENE, CODING FOR SYNTHESIS OF BOTH SOLUBLE AND INSOLUBLE GLUCANS [J].

HANADA, N ;

KURAMITSU, HK .

INFECTION AND IMMUNITY, 1988, 56 (08) :1999-2005

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