NUCLEOTIDE-SEQUENCE AND GENOME ORGANIZATION OF TOMATO LEAF CURL GEMINIVIRUS

被引：177

作者：

DRY, IB ^{[1
]}

RIGDEN, JE ^{[1
]}

KRAKE, LR ^{[1
]}

MULLINEAUX, PM ^{[1
]}

REZAIAN, MA ^{[1
]}

机构：

[1] CSIRO,DIV HORT,GPO BOX 350,ADELAIDE,SA 5001,AUSTRALIA

来源：

JOURNAL OF GENERAL VIROLOGY | 1993年 / 74卷

关键词：

D O I：

10.1099/0022-1317-74-1-147

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genome of tomato leaf curl virus (TLCV) from Australia was cloned and its complete nucleotide sequence determined. It is a single circular ssDNA of 2766 nucleotides containing the consensus nona-nucleotide sequence present in all geminiviruses. It has six open reading frames with an organization resembling that of certain other dicotyledonous plant-infecting monopartite geminiviruses, i.e. tomato yellow leaf curl and beet curly top viruses. The regulatory sequences present indicate a bidirectional mode of transcription. A dimeric TLCV DNA clone was constructed in a binary vector and used to agroinoculate three different host species. Typical virus infections were produced, confirming that the single DNA component is sufficient for infectivity.

引用

页码：147 / 151

页数：5

共 23 条

[1] MUTATIONAL ANALYSIS OF THE VIRION-SENSE GENES OF MAIZE STREAK VIRUS [J].