NOVEL MUTATION (V75T) IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE-TRANSCRIPTASE CONFERS RESISTANCE TO 2',3'-DIDEHYDRO-2',3'-DIDEOXYTHYMIDINE IN CELL-CULTURE

被引：122

作者：

LACEY, SF ^{[1
]}

LARDER, BA ^{[1
]}

机构：

[1] WELLCOME RES LABS,ANTIVIRAL THERAPEUT RES UNIT,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1994年 / 38卷 / 06期

关键词：

D O I：

10.1128/AAC.38.6.1428

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have selected a human immunodeficiency virus type 1 (HIV-1) mutant strain with a moderate (sevenfold) level of resistance to the nucleoside analog 2',3'-didehydro-2',3'-dideoxythymidine (D4T or stavudine). After serial passage of the HXB2 strain of HIV-1 in MT4 cells, a novel mutation involving two nucleotide substitutions in codon 75 of the viral reverse transcriptase, altering valine to threonine, was seen. When introduced into a wild-type HIV-1 background by site-directed mutagenesis, the T-75 mutation conferred cross-resistance to the dideoxynucleosides dideoxyinosine and dideoxycytosine as well as to 2',3'-didehydro-2',3'-dideoxycytosine.

引用

页码：1428 / 1432

页数：5

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