PRODUCTION OF HUMAN P53 SPECIFIC MONOCLONAL-ANTIBODIES AND THEIR USE IN IMMUNOHISTOCHEMICAL STUDIES OF TUMOR-CELLS

被引：0
作者：
LEGROS, Y
LACABANNE, V
DAGAY, MF
LARSEN, CJ
PLA, M
SOUSSI, T
机构：
[1] INSERM,U301,INST GENET MOLEC,27 RUE JULIETTE DODU,F-75010 PARIS,FRANCE
[2] HOP ST LOUIS,INSERM,U93,F-75010 PARIS,FRANCE
[3] HOP ST LOUIS,ANAT PATHOL LAB,F-75010 PARIS,FRANCE
来源：
BULLETIN DU CANCER | 1993年 / 80卷 / 02期
关键词：
TUMOR SUPPRESSOR GENE P53; MONOCLONAL ANTIBODIES; IMMUNOHISTOCHEMISTRY;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We describe the production of 13 new monoclonal antibodies induced by the product of the human p53 tumor suppressor gene. All these monoclonal antibodies recognize human p53 irrespective of the detection technique: ELISA, immunoblot or immunoprecipitation. These antibodies can be divided into four groups according to such criteria as localization of the recognized epitopes and reactivity with p53 originating from other organisms. One monoclonal antibody was successfully used for immunohistochemistry detection of p53 accumulation in breast carcinoma. This novel panel of monoclonal antibodies represents a further tool for the study of the p53 protein.
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页码：102 / 110
页数：9
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