HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-I PROVIRUS IS DEMONSTRATED IN PERIPHERAL-BLOOD MONOCYTES INVIVO - A STUDY UTILIZING AN INSITU POLYMERASE CHAIN-REACTION

被引：45
作者：
BAGASRA, O
POMERANTZ, RJ
机构：
[1] Infectious Disease Division, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia
来源：
AIDS RESEARCH AND HUMAN RETROVIRUSES | 1993年 / 9卷 / 01期
关键词：
D O I：
10.1089/aid.1993.9.69
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Human immunodeficiency virus type 1 (HIV-1) infects a variety of cell types in vivo. Monocyte/macrophages may be the major reservoir for HIV-1 in the solid tissues of HIV-1-infected individuals. Conflicting data have been reported, though, regarding the presence of HIV-1 provirus in peripheral blood monocytes isolated from HIV-1-seropositive humans. We have evaluated monocytes from the peripheral blood of eleven HIV-1-infected individuals utilizing a new, highly sensitive and specific in situ polymerase chain reaction. We demonstrate HIV-1 provirus in 73% (8/11) of these samples. None of these monocyte samples was demonstrated to contain cells expressing high levels of HIV-1-specific RNA, by standard in situ hybridization. The evaluation of the HIV-1 genome in peripheral blood monocytes of certain infected individuals may assist in the understanding of HIV-1 proviral latency and pathogenesis, in vivo.
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页码：69 / 76
页数：8
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