NMR REINVESTIGATION OF 2 N-ACETYLNEURAMINIC ACID-CONTAINING O-SPECIFIC POLYSACCHARIDES (O56 AND O24) OF ESCHERICHIA-COLI

被引:11
作者
TORGOV, VI [1 ]
SHASHKOV, AS [1 ]
JANN, B [1 ]
JANN, K [1 ]
机构
[1] MAX PLANCK INST IMMUNBIOL, D-79108 FREIBURG, GERMANY
关键词
D O I
10.1016/0008-6215(95)00041-Q
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Structures for the N-acetylneuraminic acid (Neu5Ac)-containing O56 and O24 polysaccharides of Escherichia coli have been reported previously. During these studies unusual chemical shifts had been observed for the NMR signals for H-3eq and C-3 of the Neu5Ac residues of both polysaccharides. In further pursuing this phenomenon, we have reinvestigated the O56 and O24 polysaccharides as well as derived oligosaccharides by one- and two-dimensional NMR spectroscopy. The results showed that structures of both polysaccharides (PSs) had to be modified and formulated as [GRAPHICS] 2D ROESY spectra revealed a strong NOE between H-3eq of Neu5Ac and the protons of the side-chain sugar (H-3 and H-5 of alpha-D-Gal p in the O56 PS and H-3 of alpha-D-Glc p in the O24 PS) and also between H-3ax of Neu5Ac and H-3 of beta-D-Glc p in the main chain. This indicated aclose spatial apposition of the seven-linked alpha-Neu5Ac and the side-chain residues alpha-D-Gal p (O56 PS) and alpha-D-Glc p (O24 PS), respectively. The strong long-range spatial contacts caused the unusual chemical shifts of H-3eq and C-3 of Neu5Ac.
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页码:73 / 90
页数:18
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