GUIDED OUTGROWTH OF LEECH NEURONS IN CULTURE

被引：41
作者：
FROMHERZ, P
SCHADEN, H
VETTER, T
机构：
来源：
NEUROSCIENCE LETTERS | 1991年 / 129卷 / 01期
关键词：
LEECH; NEURITE; GUIDANCE; ARBORIZATION; GROWTH CONE; LAMININ;
D O I：
10.1016/0304-3940(91)90724-8
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Sensory neurons of Hirudo medicinalis were cultivated on patterns of extracellular matrix (ECM) protein which were prepared by UV irradiation using copper grid masks. The relation of the patterns and of the outgrowth of neurites was observed by scanning electronmicroscopy after gold decoration. The neurites were guided by narrow (10-mu-M) lanes of ECM protein. Branching of neurites was induced by branched lanes. Bent neurites were pulled off bent lanes at some distance behind the growth cone such that their length was reduced. Regularly spaced sites of adhesion became visible which remained connected to the neurites by extended filaments.
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页码：77 / 80
页数：4
相关论文
共 12 条
[1]  
CHIQUET M, 1987, J EXP BIOL, V132, P191
[2]   ATTACHMENT TO AN ENDOGENOUS LAMININ-LIKE PROTEIN INITIATES SPROUTING BY LEECH NEURONS [J].
CHIQUET, M ;
MASUDANAKAGAWA, L ;
BECK, K .
JOURNAL OF CELL BIOLOGY, 1988, 107 (03) :1189-1198
[3]   VOLTAGE DEPENDENCE OF 5-HYDROXYTRYPTAMINE RELEASE AT A SYNAPSE BETWEEN IDENTIFIED LEECH NEURONS IN CULTURE [J].
DIETZEL, ID ;
DRAPEAU, P ;
NICHOLLS, JG .
JOURNAL OF PHYSIOLOGY-LONDON, 1986, 372 :191-&
[4]   ENERGY-TRANSFER BETWEEN FLUORESCENT DYES SPACED BY MULTILAYERS OF CADMIUM SALTS OF FATTY-ACIDS [J].
FROMHERZ, P ;
REINBOLD, G .
THIN SOLID FILMS, 1988, 160 (1-2) :347-353
[5]  
FROMHERZ P, IN PRESS SCIENCE
[6]   GROWTH CONE GUIDANCE BY SUBSTRATE-BOUND LAMININ PATHWAYS IS CORRELATED WITH NEURON-TO-PATHWAY ADHESIVITY [J].
HAMMARBACK, JA ;
MCCARTHY, JB ;
PALM, SL ;
FURCHT, LT ;
LETOURNEAU, PC .
DEVELOPMENTAL BIOLOGY, 1988, 126 (01) :29-39
[7]   GUIDANCE OF NEURITE OUTGROWTH BY PATHWAYS OF SUBSTRATUM-ADSORBED LAMININ [J].
HAMMARBACK, JA ;
PALM, SL ;
FURCHT, LT ;
LETOURNEAU, PC .
JOURNAL OF NEUROSCIENCE RESEARCH, 1985, 13 (1-2) :213-220
[8]   CIRCUITS CONSTRUCTED FROM IDENTIFIED APLYSIA NEURONS EXHIBIT MULTIPLE PATTERNS OF PERSISTENT ACTIVITY [J].
KLEINFELD, D ;
RACCUIABEHLING, F ;
CHIEL, HJ .
BIOPHYSICAL JOURNAL, 1990, 57 (04) :697-715
[9]  
KRAMER AP, 1985, J NEUROSCI, V5, P768
[10]   INVITRO RECONSTRUCTION OF THE RESPIRATORY CENTRAL PATTERN GENERATOR OF THE MOLLUSK LYMNAEA [J].
SYED, NI ;
BULLOCH, AGM ;
LUKOWIAK, K .
SCIENCE, 1990, 250 (4978) :282-285
12