ANTI-LYMPHOCYTE SERUM PRODUCED BY IMMUNIZATION WITH PURIFIED MOUSE THYMOCYTE PLASMA-MEMBRANE

被引：1

作者：

EDWARDS, AJ

机构：

[1] Clinical Research Centre, Harrow, Middlesex HA1 3UJ, Watford Road

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1979年 / 27卷 / 03期

关键词：

D O I：

10.1016/0022-1759(79)90220-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A predominantly plasma membrane fraction was isolated from mouse thymocytes and characterized. Immunization of rabbits with this material yielded highly potent antilymphocyte sera as measured by prolongation of skin allograft survival. Potency was maintained after repeated boosting of the rabbits, yet the generation of irrelevant antibodies was contained. Binding of antilymphocyte sera to lymphocytes was studied by immuno-fluorescence using the analytical facility of a fluorescence activated cell sorter (FACS-1). The results demonstrated that these anti-membrane sera recognise antigens common to thymocytes and spleen lymphocytes, bind more to T-cells and thymocytes than to B-cells and bind more avidly than antisera raised with intact cells. It was concluded that this thymocyte plasma membrane preparation is highly effective in raising powerful antilymphocyte serum. © 1979.

引用

页码：225 / 239

页数：15

共 37 条

[1] LOCATION OF NUCLEOTIDE PYROPHOSPHATASE AND ALKALINE PHOSPHODIESTERASE ACTIVITIES ON LYMPHOCYTE SURFACE-MEMBRANE [J].