IDENTIFICATION OF THE ALLERGEN PSI-C-2 FROM THE BASIDIOMYCETE PSILOCYBE-CUBENSIS AS A FUNGAL CYCLOPHILIN

被引:37
作者
HORNER, WE
REESE, G
LEHRER, SB
机构
[1] Section of Allergy and Clinical Immunology, Department of Medicine, Tulane University School of Medicine, New Orleans, LA
关键词
ALLERGENS; FUNGI; IGE; CYCLOPHILIN; PSILOCYBE CUBENSIS; BASIDIOMYCETE;
D O I
10.1159/000237007
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Basidiospores are a prevalent and frequent cause of respiratory allergies, yet their allergens remain poorly defined; thus, we have attempted a molecular characterization of representative basidiomycete allergens. A Psilocybe cubensis mycelial cDNA library was immunoscreened with patient serum. A clone was isolated that expressed a 23-kD recombinant allergen as a fusion protein and inhibited a 16-kD band (Psi c 2) in immunoprints of P cubensis extract, indicating antigenic identity, Sequence (cDNA) analysis of the clone indicates homology with cyclophilin and the deduced amino acid sequence of Psi c 2 showed 78% identity and 4% similarity with the amino acid sequence of Schizosaccharomyces pombe cyclophilin. This recombinant allergen is a useful model for epitope analysis of basidiospore allergens and fungal allergen cross-reactivity, and may provide an improved reagent for basidiospore allergy diagnosis and treatment.
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页码:298 / 300
页数:3
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