NEW REAGENTS FOR SPECTRAL TITRATION OF ACTIVE-SITE OF CHYMOTRYPSIN

被引：0

作者：

PARK, HS ^{[1
]}

LEE, S ^{[1
]}

SUH, J ^{[1
]}

机构：

[1] SEOUL NATL UNIV,DEPT CHEM,SEOUL 151742,SOUTH KOREA

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1993年 / 27卷 / 03期

关键词：

CHYMOTRYPSIN; ACTIVE-SITE TITRATION; VISIBLE REGION;

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The reaction of alpha-chymotrypsin (ChT) with 2-phenyl-(E)-[4-(E)-cinnamylidene]oxazolin-5-one (E-PCO) or 2-phenyl-(Z)-[4-(E)-cinnamylidene]oxazolin-5-one (Z-PCO) at pH 4.5 and 25-degrees-C led to the quantitative accumulation of the corresponding acyl-ChT intermediate. Very large changes in molar extinction coefficient were observed in the visible region during the conversion of the oxazolinones to the acyl-ChT intermediates. This afforded a reliable method for the spectral titration of ChT in the visible region. Both E-PCO and Z-PCO were stable under these conditions and did not cause any complication in the titration of the active site.

引用

页码：261 / 265

页数：5

共 11 条

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