CAPILLARY ELECTROPHORESIS WITH AMPEROMETRIC DETECTION USING A POROUS CELLULOSE-ACETATE JOINT

被引：48
作者：
CHEN, IC [1 ]
WHANG, CW [1 ]
机构：
[1] TUNGHAI UNIV,DEPT CHEM,TAICHUNG 40704,TAIWAN
来源：
JOURNAL OF CHROMATOGRAPHY | 1993年 / 644卷 / 01期
关键词：
D O I：
10.1016/0021-9673(93)80133-S
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A system for coupling amperometric detection with capillary electrophoresis (CE) is described. The interface is formed by fracturing the capillary followed by covering the fracture with a thin layer of cellulose acetate (CA) film. The porous CA joint enables off-column amperometric detection to be performed without adverse effects from the high electrophoretic voltage. The primary advantage of this CA joint is its simple construction. The performance of the CE-amperometric detection system was evaluated with hydroquinone. A detection limit of 8 . 10(-8) M or 0. 17 fmol with a theoretical plate number exceeding 90 000 was obtained. Application of the system to the separation and detection of aminophenols, catecholamines and catechol was also demonstrated.
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页码：208 / 212
页数：5
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