THE USE OF POLYCLONAL ACTIVATORS IN THE PRODUCTION OF MURINE MONOCLONAL AND POLYCLONAL ANTIBODIES

被引：2

作者：

FIORI, PL ^{[1
]}

RAPPELLI, P ^{[1
]}

机构：

[1] CNR,IST GENET MOLEC,PORTO CONTE RES LABS,SASSARI,ITALY

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 139卷 / 02期

关键词：

POLYCLONAL ACTIVATOR; MONOCLONAL ANTIBODY; PROTEIN PURIFIED DERIVATIVE;

D O I：

10.1016/0022-1759(91)90187-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We propose a new immunization method to stimulate a strong immune response against weak or diluted antigens. This technique is based on stimulation with polyclonal activators before exposure to the antigens. We also discuss the efficiency of various types of mitogen with particular regard to their capacity to produce monoclonal antibodies and serum antibodies. A specific immune response against soluble antigens is increased by pretreating mice with PPD. This preactivation permitted us to obtain monoclonal antibodies against weak antigens in a few days. No monoclonal antibodies were obtained by inoculating weak antigens or the activators by themselves.

引用

页码：181 / 190

页数：10

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