OPTIMIZATION OF ALPHA-AMYLASE AND GLUCOAMYLASE PRODUCTION BY RECOMBINANT STRAINS OF SACCHAROMYCES-CEREVISIAE

被引：9
作者：
DAGUANNO, VS
PRETORIUS, IS
机构：
[1] UNIV STELLENBOSCH,DEPT MICROBIOL,STELLENBOSCH 7600,SOUTH AFRICA
[2] UNIV STELLENBOSCH,INST BIOTECHNOL,STELLENBOSCH 7600,SOUTH AFRICA
来源：
BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 07期
关键词：
D O I：
10.1007/BF00136479
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Replacement of the regulatory sequence of the Bacillus amyloliquefaciens alpha-amylase gene (AMY1) by the yeast alcohol dehydrogenase gene promoter (ADC1(P)) resulted in increased levels of extracellular alpha-amylase production in Saccharomyces cerevisiae. Negative regulation of glucoamylase synthesis by the STA10-encoded repressor was alleviated by replacing the native STA2 gene promoter from S. cerevisiae var. diastaticus with ADC1(P). Enhanced degradation of starch was achieved when the modified versions of the AMY1 and STA2 genes were introduced jointly into S. cerevisiae.
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页码：727 / 732
页数：6
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