HOW TO PREDICT PRODUCT YIELD IN KINETICALLY CONTROLLED ENZYMATIC PEPTIDE-SYNTHESIS

被引：15
作者：
GOLOLOBOV, MY
KOZLOVA, EV
BORISOV, IL
SCHELLENBERGER, U
SCHELLENBERGER, V
JAKUBKE, HD
机构：
[1] MV LOMONOSOV STATE UNIV,BELOZERSKY INST PHYS CHEM BIOL,MOSCOW 119899,USSR
[2] KARL MARX UNIV LEIPZIG,DIV BIOSCI,DEPT BIOCHEM,O-7010 LEIPZIG,GERMANY
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 40卷 / 03期
关键词：
THEORY OF ENZYMATIC PEPTIDE SYNTHESIS; PROGRESS CURVE KINETICS; PAPAIN-CATALYZED PEPTIDE SYNTHESIS;
D O I：
10.1002/bit.260400313
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters-alpha and beta for papain-catalyzed synthesis of Mal-L-Phe-L-Ala-L-LeuNH2 from Mal-L-Phe-L-AlaOMe and L-LeuNH2. The experimental results demonstrate that this theory adequately describes the real process.
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页码：432 / 436
页数：5
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