ENHANCED HEMATOPOIETIC ACTIVITY OF A HUMAN GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR INTERLEUKIN-3 FUSION PROTEIN

被引：158
作者：
CURTIS, BM
WILLIAMS, DE
BROXMEYER, HE
DUNN, J
FARRAH, T
JEFFERY, E
CLEVENGER, W
DEROOS, P
MARTIN, U
FRIEND, D
CRAIG, V
GAYLE, R
PRICE, V
COSMAN, D
MARCH, CJ
PARK, LS
机构：
[1] IMMUNEX RES & DEV CORP,51 UNIV ST,SEATTLE,WA 98101
[2] INDIANA UNIV,SCH MED,WALTHER ONCOL CTR,INDIANAPOLIS,IN 46202
[3] INDIANA UNIV,SCH MED,DEPT MED,INDIANAPOLIS,IN 46202
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 13期
关键词：
GROWTH FACTORS; HEMATOPOIESIS;
D O I：
10.1073/pnas.88.13.5809
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Granulocyte/macrophage colony-stimulating factor-interleukin 3 (GM-CSF-IL-3) fusion proteins were generated by construction of a plasmid in which the coding regions of human GM-CSF and IL-3 cDNAs were connected by a synthetic linker sequence followed by subsequent expression in yeast. Both GM-CSF-IL-3 and IL-3-GM-CSF fusion proteins were purified to homogeneity and shown to bind to cell-surface receptors through either their GM-CSF or IL-3 domains. The fusion proteins exhibited enhanced receptor affinity, proliferative activity, and hematopoietic colony-stimulating activity compared with either IL-3 and/or GMCSF alone. This suggests that GM-CSF-IL-3 fusion proteins may hold future promise as therapeutic agents.
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收藏
页码：5809 / 5813
页数：5
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