MOLECULAR-CLONING OF THE LARGE SUBUNIT OF TRANSFORMING GROWTH-FACTOR TYPE-BETA MASKING PROTEIN AND EXPRESSION OF THE MESSENGER-RNA IN VARIOUS RAT-TISSUES

被引：145

作者：

TSUJI, T

OKADA, F

YAMAGUCHI, K

NAKAMURA, T

机构：

[1] KYUSHU UNIV,FAC SCI,DEPT BIOL,FUKUOKA 812,JAPAN

[2] KYOWA HAKKO KOGYO CO LTD,TOKYO RES LABS,MACHIDA,TOKYO 194,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 22期

关键词：

binding protein; cysteine-rich internal repeat; epidermal growth factor-like domain; primary structure;

D O I：

10.1073/pnas.87.22.8835

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Masking protein (MP), which neutralizes the activity of transforming growth factor type β1 (TGF-β1), is composed of a dimeric N-terminal part of a TGF-β1 precursor of M(r) 39,000 and an unknown large subunit of M(r) 105,000-120,000. The deduced primary structure of the MP large subunit was elucidated by determining the nucleotide sequence of its cDNA. The cDNA encodes a prepro-precursor of 1712 amino acid residues with a calculated M(r) of 186,596. The mature large subunit seems to be derived proteolytically from a prepro-precursor and the calculated M(r) is 91,606. The precursor has seven N-linked glycosylation sites and an unusual structure containing 18 epidermal growth factor-like domains and four cysteine-rich internal repeats. The large subunit mRNA is synthesized in parallel with the expression of TGF-β1 mRNA in various rat tissues.

引用

页码：8835 / 8839

页数：5

共 28 条

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