PROPERTIES OF THE NA+/H+ ANTIPORTER IN VIBRIO-PARAHAEMOLYTICUS

被引：21
|
作者：
KURODA, T [1 ]
SHIMAMOTO, T [1 ]
INABA, K [1 ]
KAYAHARA, T [1 ]
TSUDA, M [1 ]
TSUCHIYA, T [1 ]
机构：
[1] OKAYAMA UNIV,FAC PHARMACEUT SCI,DEPT MICROBIOL,OKAYAMA 700,JAPAN
来源：
JOURNAL OF BIOCHEMISTRY | 1994年 / 115卷 / 06期
关键词：
NA+/H+ ANTIPORTER; PH-DEPENDENCE; PROPERTIES; SUBSTRATE SPECIFICITY; VIBRIO PARAHAEMOLYTICUS;
D O I：
10.1093/oxfordjournals.jbchem.a124473
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The properties of the Na+/H+ antiporter in Vibrio parahaemolyticus, a slightly halophilic bacterium, were investigated using everted membrane vesicles. It appears that at least two Na+/H+ antiporters are present, one that is pH-dependent and one that is pH-independent. These two antiporters appear to correspond to the NhaA and NhaB antiporters of Escherichia coli, respectively. It seems that amiloride strongly inhibits the pH-dependent antiporter. Na+ is the best substrate for both of the two V. parahaemolyticus antiporters. Li+ is a poorer substrate and K+ is not a substrate. No K+/H+ antiport activity was detected in membrane vesicles of this organism. The Na+(Li+)/H+ antiport activity greatly increased with an increase in pH of the assay medium. pH did not affect the K-m value of the Na+/H+ antiport, but it did increase the V-max.
引用
收藏
页码：1162 / 1165
页数：4
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