PH-DEPENDENCE OF LIGHT-INDUCED PROTON RELEASE BY BACTERIORHODOPSIN

被引：56
作者：
KONO, M
MISRA, S
EBREY, TG
机构：
[1] UNIV ILLINOIS,BIOPHYS PROGRAM,156 DAVENPORT HALL,URBANA,IL 61801
[2] UNIV ILLINOIS,DEPT CELL & STRUCT BIOL,URBANA,IL 61801
来源：
FEBS LETTERS | 1993年 / 331卷 / 1-2期
关键词：
PURPLE MEMBRANE; PROTON PUMP;
D O I：
10.1016/0014-5793(93)80291-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have measured the current generated by light-activated proton release from bacteriorhodopsin into solution as a function of both pH and ionic strength. We find that proton release into solution decreases with increasing pH with an intrinsic pk(a) of 8.2 +/- 0.2. This pH dependence indicates that the deprotonation of a certain group inhibits or abolishes proton release. Under physiological conditions, this group either releases a proton directly into solution or interacts with the site of proton release. The most immediate candidates for this protonatable species are tyrosine-57, tyrosine-185, arginine-82, and water; acting individually or cooperatively. The salt dependence of the apparent pK(a) of this group also allows us to calculate the surface charge density of about -5 charges per bacteriorhodopsin, compatible with previous estimates.
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页码：31 / 34
页数：4
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