SATURATION MUTAGENESIS OF THE UASNTR (GATAA) RESPONSIBLE FOR NITROGEN CATABOLITE REPRESSION-SENSITIVE TRANSCRIPTIONAL ACTIVATION OF THE ALLANTOIN PATHWAY GENES IN SACCHAROMYCES-CEREVISIAE

被引：63

作者：

BYSANI, N ^{[1
]}

DAUGHERTY, JR ^{[1
]}

COOPER, TG ^{[1
]}

机构：

[1] UNIV TENNESSEE,CTR HLTH SCI,DEPT MICROBIOL & IMMUNOL,MEMPHIS,TN 38163

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 16期

关键词：

D O I：

10.1128/jb.173.16.4977-4982.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Saturation mutagenesis of the UAS(NTR) element responsible for GLN3-dependent, nitrogen catabolite repression-sensitive transcriptional activation of allantoin pathway genes in yeast cells identified the dodecanucleotide sequence 5'-TTNCTGATAAGG-3' as the minimum required for UAS activity. There was significant flexibility in mutant sequences capable of supporting UAS activity, which correlates well with the high variation in UAS(NTR) homologous sequences reported to be upstream of the DAL and DUR genes. Three of nine UAS(NTR)-like sequences 5' of the DAL5 gene supported high-level transcriptional activation. The others, which contained nonpermissive substitutions, were not active.

引用

页码：4977 / 4982

页数：6

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