EFFECT OF RAT CINC/GRO, A MEMBER OF THE INTERLEUKIN-8 FAMILY, ON LEUKOCYTES IN MICROCIRCULATION OF THE RAT MESENTERY

被引：80

作者：

WATANABE, K ^{[1
]}

SUEMATSU, M ^{[1
]}

IIDA, M ^{[1
]}

TAKAISHI, K ^{[1
]}

IIZUKA, Y ^{[1
]}

SUZUKI, H ^{[1
]}

SUZUKI, M ^{[1
]}

TSUCHIYA, M ^{[1
]}

TSURUFUJI, S ^{[1
]}

机构：

[1] KEIO UNIV,SCH MED,DEPT INTERNAL MED,SHINJUKU KU,TOKYO 160,JAPAN

来源：

EXPERIMENTAL AND MOLECULAR PATHOLOGY | 1992年 / 56卷 / 01期

关键词：

D O I：

10.1016/0014-4800(92)90023-5

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Rat cytokine-induced neutrophil chemoattractant (CINC) is a member of the IL-8 family, and its human counterpart is gro/MGSA but not IL-8. We ascertained that chemically synthesized CINC was comparable to native CINC/gro with regard to chemotactic activity for rat neutrophils and studied the effect of synthesized CINC/gro on circulating leukocytes in microvascular vessels of rat mesentery. Exposure of rat mesentery to 10-8 M authentic CINC/gro induced neutrophil adherence to and extravasation from postcapillary venules (PCVs) but not from capillaries or arterioles. CINC/gro concentrations as low as 10-10 M were effective in causing neutrophil adherence. Neutrophils adhered to thin PCVs (mean diameter, ∼25 μm) after exposure to CINC/gro for 15 min. The mean diameters of the PCV with adherence of neutrophils after exposure to CINC/gro for 30 and 60 min were 37 and 43 μm, respectively. The diameters of PCV with extravasation of neutrophils also increased in a time-dependent manner. The starting position of adherence of neutrophils was ∼25-50 μm away from the upper junction of two vessels and remained virtually unchanged during exposure to CINC/gro for 60 min. However, the distance from the start to the end of neutrophil adherence increased in a time-dependent manner. The effect of CINC/gro on adherence and extravasation of leukocytes was neutrophil specific since other leukocytes such as lymphocytes and monocytes were not identified among the adherent and extravasated leukocytes. © 1992.

引用

页码：60 / 69

页数：10

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