PURIFICATION OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE FROM RAT-LIVER

被引：50
作者：
NESS, GC
SPINDLER, CD
MOFFLER, MH
机构：
[1] Department of Biochemistry, College of Medicine, University of South Florida, Tampa
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 197卷 / 02期
关键词：
D O I：
10.1016/0003-9861(79)90272-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A procedure for the purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase [mevalonate:NADP+ oxidoreductase (CoA-acylating); EC 1.1.1.34] from rat liver microsomes has been developed. The enzyme preparations obtained by this procedure have specific activities of 16 to 23 μmol of mevalonate formed per minute per milligram of protein. These enzyme preparations were judged to be homogeneous on the basis of comigration of enzyme activity and protein on polyacrylamide gels. © 1979.
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页码：493 / 499
页数：7
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