PURIFICATION OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A REDUCTASE FROM RAT-LIVER

被引：50
作者：
NESS, GC
SPINDLER, CD
MOFFLER, MH
机构：
[1] Department of Biochemistry, College of Medicine, University of South Florida, Tampa
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 197卷 / 02期
关键词：
D O I：
10.1016/0003-9861(79)90272-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A procedure for the purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase [mevalonate:NADP+ oxidoreductase (CoA-acylating); EC 1.1.1.34] from rat liver microsomes has been developed. The enzyme preparations obtained by this procedure have specific activities of 16 to 23 μmol of mevalonate formed per minute per milligram of protein. These enzyme preparations were judged to be homogeneous on the basis of comigration of enzyme activity and protein on polyacrylamide gels. © 1979.
引用
收藏
页码：493 / 499
页数：7
相关论文
共 22 条
[1]  
AVIGAN J, 1975, J LIPID RES, V16, P151
[2]   ACTIVATION OF PURIFIED 3-HYDROXY-3-METHYLGLUTARYL-COA REDUCTASE BY PHOSPHOLIPIDS [J].
BERDE, CB ;
HELLER, RA ;
SIMONI, RD .
BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 488 (01) :112-120
[3]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4]   PROTEIN-CONCENTRATION OF CRUDE CELL AND TISSUE-EXTRACTS AS ESTIMATED BY THE METHOD OF DYE BINDING - COMPARISON WITH THE LOWRY METHOD [J].
CHIAPPELLI, F ;
VASIL, A ;
HAGGERTY, DF .
ANALYTICAL BIOCHEMISTRY, 1979, 94 (01) :160-165
[5]   PHOSPHOENOLPYRUVATE CARBOXYKINASE (GUANOSINE TRIPHOSPHATE) FROM RAT-LIVER CYTOSOL - SEPARATION OF HOMOGENEOUS FORMS OF ENZYME WITH HIGH AND LOW ACTIVITY BY CHROMATOGRAPHY ON AGAROSE-HEXANE-GUANOSINE TRIPHOSPHATE [J].
COLOMBO, G ;
CARLSON, GM ;
LARDY, HA .
BIOCHEMISTRY, 1978, 17 (25) :5321-5329
[6]  
DIETSCHY JM, 1974, J LIPID RES, V15, P508
[7]  
EDWARDS PA, 1979, J LIPID RES, V20, P40
[8]  
EICHLER DC, 1973, THESIS U CALIFORNIA
[9]  
HELLER RA, 1976, J BIOL CHEM, V251, P3815
[10]   PREVENTION OF COLD INACTIVATION OF 3-HYDROXY-3-METHYLGLUTARYL COENZYME A REDUCTASE BY NADPH [J].
HELLER, RA ;
GOULD, RG .
BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 388 (02) :254-259
123