RYANODINE-SENSITIVE CALCIUM-RELEASE CHANNEL FROM LEFT-VENTRICLE, SEPTUM, AND ATRIUM OF CANINE HEART

被引：14
作者：
XU, L
COHN, AH
MEISSNER, G
机构：
[1] UNIV N CAROLINA,DEPT CHEM,CB 7260,CHAPEL HILL,NC 27599
[2] UNIV N CAROLINA,DEPT BIOPHYS,CHAPEL HILL,NC 27599
[3] UNIV N CAROLINA,DEPT PHYSIOL,CHAPEL HILL,NC 27599
来源：
CARDIOVASCULAR RESEARCH | 1993年 / 27卷 / 10期
关键词：
D O I：
10.1093/cvr/27.10.1815
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Objective: The aim was to consider the possibility that functionally distinct forms of the ryanodine sensitive calcium release channel are expressed in different regions of heart. Methods: Membranous fractions enriched in ryanodine binding activity were isolated from canine left ventricular free wall, interventricular septum, and atrium. Ryanodine receptors (RyR) were purified by sucrose density gradient centrifugation, following solubilisation of sarcoplasmic reticular membranes with the detergent Chaps. Single channel currents were measured, upon reconstitution of sarcoplasmic reticular vesicles and the purified RyR into planar lipid bilayers. Results: Ryanodine sensitive Ca2+ release channels from three different regions of canine heart displayed the same [H-3]ryanodine binding and single channel characteristics. Conclusions: The left ventricular free wall, septum, and atrium of canine heart may express functionally related, if not identical, ryanodine receptor/Ca2+ release channels.
引用
收藏
页码：1815 / 1819
页数：5
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