MOLECULAR CHARACTERIZATION OF THE INTERLEUKIN-8 RECEPTOR

被引：40

作者：

BECKMANN, MP ^{[1
]}

MUNGER, WE ^{[1
]}

KOZLOSKY, C ^{[1
]}

VANDENBOS, T ^{[1
]}

PRICE, V ^{[1
]}

LYMAN, S ^{[1
]}

GERARD, NP ^{[1
]}

GERARD, C ^{[1
]}

CERRETTI, DP ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,BOSTON,MA 02115

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91885-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently a rabbit cDNA (F3R) was characterized as binding and causing calcium mobilization induced by the formyl-methionine-leucine-phenylalanine peptide (fMLP). In the study reported here, cloned DNAs were isolated from rabbit genomic DNA by PCR based on the sequence of F3R. The cloned DNAs have several differences in the DNA sequence compared to the reported F3R sequence that alter the predicted protein sequence. COS-7 cells transfected with these clones in a mammalian expression vector bind human IL-8 with high affinity, but do not bind fMLP. We therefore believe that the cDNAs isolated encode the rabbit IL-8 receptor. © 1991.

引用

收藏

页码：784 / 789

页数：6

相关论文

共 21 条

[1] CONSTITUTIVE OVEREXPRESSION OF A GROWTH-REGULATED GENE IN TRANSFORMED CHINESE-HAMSTER AND HUMAN-CELLS [J].

ANISOWICZ, A ;

BARDWELL, L ;

SAGER, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :7188-7192

[2]

BECKMANN MP, 1990, J IMMUNOL, V144, P4212

[3] SYNTHESIS AND USE OF A NOVEL N-FORMYL PEPTIDE DERIVATIVE TO ISOLATE A HUMAN N-FORMYL PEPTIDE RECEPTOR CDNA [J].

BOULAY, F ;

TARDIF, M ;

BROUCHON, L ;

VIGNAIS, P .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 168 (03) :1103-1109

[4] A NEW SIMPLIFIED SINGLE-FILTER ASSAY FOR INVITRO EVALUATION OF CHEMOTAXIS OF CR-51-LABELED POLYMORPHONUCLEAR LEUKOCYTES [J].

CAPSONI, F ;

MINONZIO, F ;

ONGARI, AM ;

ZANUSSI, C .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 120 (01) :125-131

[5] HUMAN MACROPHAGE-COLONY STIMULATING FACTOR - ALTERNATIVE RNA AND PROTEIN PROCESSING FROM A SINGLE GENE [J].

CERRETTI, DP ;

WIGNALL, J ;

ANDERSON, D ;

TUSHINSKI, RJ ;

GALLIS, BM ;

STYA, M ;

GILLIS, S ;

URDAL, DL ;

COSMAN, D .

MOLECULAR IMMUNOLOGY, 1988, 25 (08) :761-770

[6] RECOMBINANT EXPRESSION, BIOCHEMICAL-CHARACTERIZATION, AND BIOLOGICAL-ACTIVITIES OF THE HUMAN MGSA-GRO PROTEIN [J].

DERYNCK, R ;

BALENTIEN, E ;

HAN, JH ;

THOMAS, HG ;

WEN, DZ ;

SAMANTHA, AK ;

ZACHARIAE, CO ;

GRIFFIN, PR ;

BRACHMANN, R ;

WONG, WL ;

MATSUSHIMA, K ;

RICHMOND, A .

BIOCHEMISTRY, 1990, 29 (44) :10225-10233

[7] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[8] ENDOTHELIAL INTERLEUKIN-8 - A NOVEL INHIBITOR OF LEUKOCYTE-ENDOTHELIAL INTERACTIONS [J].

GIMBRONE, MA ;

OBIN, MS ;

BROCK, AF ;

LUIS, EA ;

HASS, PE ;

HEBERT, CA ;

YIP, YK ;

LEUNG, DW ;

LOWE, DG ;

KOHR, WJ ;

DARBONNE, WC ;

BECHTOL, KB ;

BAKER, JB .

SCIENCE, 1989, 246 (4937) :1601-1603

[9] A SHORT POLYPEPTIDE MARKER SEQUENCE USEFUL FOR RECOMBINANT PROTEIN IDENTIFICATION AND PURIFICATION [J].

HOPP, TP ;

PRICKETT, KS ;

PRICE, VL ;

LIBBY, RT ;

MARCH, CJ ;

CERRETTI, DP ;

URDAL, DL ;

CONLON, PJ .

BIO-TECHNOLOGY, 1988, 6 (10) :1204-1210

[10] NEUTROPHIL-ACTIVATING PROPERTIES OF THE MELANOMA GROWTH STIMULATORY ACTIVITY [J].

MOSER, B ;

CLARKLEWIS, I ;

ZWAHLEN, R ;

BAGGIOLINI, M .

JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 171 (05) :1797-1802