DETERMINATION OF PHLOROGLUCINOL IN HUMAN PLASMA BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：17

作者：

LARTIGUEMATTEI, C ^{[1
]}

LAUROMARTY, C ^{[1
]}

BASTIDE, M ^{[1
]}

BERGER, JA ^{[1
]}

CHABARD, JL ^{[1
]}

GOUTAY, E ^{[1
]}

AIACHE, JM ^{[1
]}

机构：

[1] FAC PHARM CLERMONT FERRAND,BIOPHARM LAB,RECH BIODYNAM MED GRP,PL HENRI DUNANT,BP 38,F-63001 CLERMONT FERRAND,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 617卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80433-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A specific and sensitive method has been developed for the determination of phloroglucinol in plasma; it involves an optimized procedure for blood sampling designed to minimize the in vitro oxidation of the molecule, and ps chromatography-mass spectrometry after silylation of the compound. The method allowed a reliable determination of phloroglucinol in plasma. The precision and accuracy of the assay, reported as coefficients of variation, were below 15%. Using a plasma sample of 0.25 ml, the limit of quantitation was 5 ng/ml with a precision of 17.4%, which is sensitive enough for pharmacokinetic studies. Stability studies under different conditions revealed that ascorbic acid limits the degradation of phloroglucinol in plasma during storage at freezer temperatures.

引用

页码：140 / 146

页数：7

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