INTRAMOLECULAR TRANSESTERIFICATION IN THIOPHOSPHATE-ANALOGS OF AN RNA-DIMER

被引：30

作者：

ALMER, H

STROMBERG, R

机构：

[1] Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University

来源：

TETRAHEDRON LETTERS | 1991年 / 32卷 / 30期

关键词：

D O I：

10.1016/S0040-4039(00)79778-4

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The differences in rates for cleavage of uridylyl(3'-5') uridine and the uridin-3'-yl uridin-5'-yl thiophosphates (R(p) and S(p)-diastereomers) have been measured. The ratios of rates are: k(phosphate)/k(R(p)-thiophosphate) = 1.3; k(phosphate)/k(S(p)-thiophosphate) = 0.78; k(S(p)-thiophosphate)/k(R(p)-thiophosphate) = 1.7 and they were found to be independent of pH in the pH-range 9-12. The results are also discussed in the context of using thiophosphates in kinetic studies of enzymatic systems involving transesterification of phosphodiesters.

引用

页码：3723 / 3726

页数：4

共 15 条

[1]

ALMER H, IN PRESS

[2]

[Anonymous], 1971, ENZYMES, V4, P313

[3] ABSOLUTE-CONFIGURATION OF DIASTEREOMERS OF ADENOSINE 5'-O-(1-THIOTRIPHOSPHATE) - CONSEQUENCES FOR STEREOCHEMISTRY OF POLYMERIZATION BY DNA-DEPENDENT RNA-POLYMERASE FROM ESCHERICHIA-COLI [J].