INFLUENCE OF METHOD FOR REMOVAL OF SESTON ON DISSOLVED ORGANIC MATTER .2. COBALAMINS

被引：4

作者：

PARKER, BC

机构：

[1] Department of Botany, Washington University, St Louis, Missouri

来源：

JOURNAL OF PHYCOLOGY | 1969年 / 5卷 / 02期

关键词：

D O I：

10.1111/j.1529-8817.1969.tb02590.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Comparisons of the 3 previously used methods (continuous flow centrifugation, Millipore®filtration, and biodialysis) for making water samples seston‐free before analysis for cobalamins point to several likely sources of error: (a) unpredictable occlusion of pores in Millipore®membranes during filtration; (b) irreversible adsorption of cobalamins by Millipore®membranes; (c) probable cell injury and leakage by centrifugation. Some water samples gave good agreement on soluble vitamin B12 by all 3 methods, while other samples had appreciable discrepancies. The validity of total vs. sestonic cobalamin determinations therefore is questioned. One lake had time‐dependent fluctuation in soluble cobalamin. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：124 / &