EFFECT OF DIETARY HYPERLIPIDEMIC COMPONENTS AND FISH-OIL ON CONCENTRATION OF LIPIDS IN LIVER AND LIVER FATTY-ACID PROFILE OF RATS

被引：10

作者：

STANGL, GI ^{[1
]}

KIRCHGESSNER, M ^{[1
]}

EDER, K ^{[1
]}

REICHLMAYRLAIS, AM ^{[1
]}

机构：

[1] TECH UNIV MUNICH,INST ERNAHRUNGSPHYSIOL,D-85350 FREISING,GERMANY

来源：

ZEITSCHRIFT FUR ERNAHRUNGSWISSENSCHAFT | 1994年 / 33卷 / 03期

关键词：

DIETARY CHOLESTEROL; SATURATED FAT; FISH OIL; LIPID CONCENTRATION; FATTY ACID COMPOSITION; LIVER; RAT;

D O I：

10.1007/BF01610785

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

This investigation was attempted to clarify the effects of dietary hyperlipidemic components and fish oil on concentration of lipids in liver and liver fatty acid profile of rats. In a first experiment male Sprague-Dawley rats were maintained on a semipurified low-fat diet with 6.5 % coconut oil and 1.5 % safflower oil, but without added cholesterol, or a high-fat hyperlipidemic diet supplemented with 7.3 % coconut oil, 7.3 % beef tallow, 0.4 % safflower oil, and 1.5 % cholesterol for 28 days. Rats fed the hyperlipidemic diet were then switched to fish oil diets with 1.4 %, 2.8 %, and 5.6 % fish oil in exchange for coconut oil and beef tallow for, respectively, 10 and 20 days. In a second experiment male Sprague-Dawley rats were fed low-fat or high-fat diets without or with 1.5 % added cholesterol for 28 days. Half of each group was then changed to a fish oil diet (for 20 days) which contained 5.6 % fish oil in exchange for coconut oil and beef tallow. In experiment 1, rats fed the hyperlipidemic diet had enlarged fatty livers within 28 days. Experiment 2 showed that cholesterol in the diet was responsible for the accumulating liver lipids. Feeding diets with added cholesterol, the proportions of saturated fatty acids (SFA), especially 18:0, were markedly reduced in liver, whereas levels of monounsaturated fatty acids (MUFA) were greatly increased compared to diets without added cholesterol. This increase was most pronounced with respect to 16:1 and 18:1. In contrast, fish oil diets towered MUFA level in liver in spite of its high MUFA content. Rats fed the highly saturated hyperlipidemic diet low in 18:2 n-6 had reduced 18: 2 n-6 levels in liver compared to rats fed the low-fat diet without added cholesterol. Also, 20: 4 n-6 level in liver was markedly reduced after the administration of the hyperlipidemic diet or the fish oil diets. Results of experiment 2 elucidate that cholesterol as well as fish oil sharply lowered 20: 4 n-6 level in liver, which might be due to a reduced desaturation. In both experiments feeding fish oil increased all long-chain n-3 polyunsaturated fatty acids (PUFA) in liver. This occurred in a dose-dependent fashion and reached a maximum level with 5.6 % fish oil in the diet. When additional cholesterol was applied 22: 6 n-3 level in liver fell. This indicates also an impaired desaturation of PUFA due to dietary cholesterol.

引用

页码：195 / 206

页数：12

共 34 条

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