Gc-Ms Study of Two Column Fractions from Methanol Extracts of Loranthus Micranthus and Their In Vivo Antidiabetic Activity on Alloxan Induced Diabetic Rats

The methanol extracts of Loranthus micranthus fractions were collected from column chromatography. From TLC, the fraction 17 and 20 were selected based on the clear distiguishable of bands and few phytochemicals. Same fractions when subjected to GC-MS, fraction-17 yielded 13 and fraction-20 yielded 11 prominent compounds. Diabetic rats that were treated with the fraction 17 and 20 showed significantly increase in body weight (16.52 and 12.43 % respectively). Hypoglycemic effect and antihyperlipidemic characters (decrease in the previously elevated levels of serum cholesterol and serum triglycerides) were observed. The two fractions treated rats have also showed the regeneration of pancreatic connective tissue, acini, blood vessels, blood capillaries, pancreatic ducts and their walls, islets capsule and cells in accordance to proper cytoarchitecture. The in vivo antidiabetic property may be due to the presence of single or all phytochemicals in the fraction. Greater activity was observed in fraction-17. However, further experimental studies are still needed for identification of the functionally relevant phytochemical(s) in management of diabetes in rats.