EXPRESSION PATTERN OF THE RAR-ALPHA-PML FUSION GENE IN ACUTE PROMYELOCYTIC LEUKEMIA

被引：150
作者：
ALCALAY, M
ZANGRILLI, D
FAGIOLI, M
PANDOLFI, PP
MENCARELLI, A
LOCOCO, F
BIONDI, A
GRIGNANI, F
PELICCI, PG
机构：
[1] UNIV PERUGIA,MONTELUCE POLICLIN,IST CLIN MED 1,I-06100 PERUGIA,ITALY
[2] UNIV ROME 2,CATTEDRA EMATOL,DIPARTIMENTO MED INTERNA,I-00100 ROME,ITALY
[3] UNIV ROME 1,DIPARTIMENTO BIOPATOL,DIV EMATOL,I-00161 ROME,ITALY
[4] UNIV MILAN,OSPED S GERARDO,PEDIAT CLIN,I-20052 MONZA,ITALY
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 11期
关键词：
RECIPROCAL TRANSLOCATION; LEUKEMOGENESIS; TRANSCRIPTION FACTORS;
D O I：
10.1073/pnas.89.11.4840
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Two chimeric genes, PML-RAR-alpha and RAR-alpha-PML, are formed as a consequence of the acute promyelocytic leukemia (APL)-specific reciprocal translocation of chromosomes 15 and 17 [t(15;17)]. PML-RAR-alpha is expressed as a fusion protein. We investigated the organization and expression pattern of the RAR-alpha-PML gene in a series of APL patients representative of the molecular heterogeneity of the t(15;17) and found (i) two types of RAR-alpha-PML mRNA junctions (RAR-alpha exon 2/PML exon 4 or RAR-alpha exon 2/PML exon 7) that maintain the RAR-alpha and PML longest open reading frames aligned and are the result of chromosome 15 breaking at two different sites; and (ii) 10 different RAR-alpha-PML fusion transcripts that differ for the assembly of their PML coding exons. A RAR-alpha-PML transcript was present in most, but not all, APL patients.
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页码：4840 / 4844
页数：5
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