TRANSGENIC MICE WITH TARGETED INACTIVATION OF THE COL2A1 GENE FOR COLLAGEN-II DEVELOP A SKELETON WITH MEMBRANOUS AND PERIOSTEAL BONE BUT NO ENDOCHONDRAL BONE

被引:195
作者
LI, SW
PROCKOP, DJ
HELMINEN, H
FASSLER, R
LAPVETELAINEN, T
KIRALY, K
PELTARRI, A
AROKOSKI, J
LUI, H
ARITA, M
KHILLAN, J
机构
[1] THOMAS JEFFERSON UNIV, JEFFERSON MED COLL, DEPT BIOCHEM & MOLEC BIOL, PHILADELPHIA, PA 19107 USA
[2] UNIV KUOPIO, DEPT ANAT, SF-70211 KUOPIO, FINLAND
[3] MAX PLANCK INST BIOCHEM, DEPT PROT CHEM, D-82152 MARTINSRIED, GERMANY
关键词
TRANSGENIC MICE; COL2A1; GENE; COLLAGEN II; CARTILAGE MATRIX; VERTEBRATE SKELETON;
D O I
10.1101/gad.9.22.2821
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Homologous recombination in embryonic stem cells was used to prepare transgenic mice with an inactivated Col2a1 gene for collagen II, the major protein component of the extracellular matrix of cartilage. Heterozygous mice had a minimal phenotype. Homozygous mice developed into fetuses that were delivered vaginally but died either just before or shortly after birth. The cartilage in the mice consisted of highly disorganized chondrocytes with a complete lack of extracellular fibrils discernible by electron microscopy. There was no endochondrial bone or epiphyseal growth plate in long bones. However, many skeletal structures such as the cranium and ribs were normally developed and mineralized. The results demonstrate that a well-organized cartilage matrix is required as a primary tissue for development of some components of the vertebrate skeleton, but it is not essential for others.
引用
收藏
页码:2821 / 2830
页数:10
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