Studying the Mechanisms of Developmental Vocal Learning and Adult Vocal Performance in Zebra Finches through Lentiviral Injection

被引:1
|
作者
Shi, Zhimin [1 ]
Tchernichovski, Ofer [2 ]
Li, XiaoChing [1 ]
机构
[1] LSU Sch Med, Neurosci Ctr Excellence, New Orleans, LA 70112 USA
[2] CUNY Hunter Coll, Dept Psychol, 695 Pk Ave, New York, NY 10021 USA
来源
BIO-PROTOCOL | 2018年 / 8卷 / 17期
基金
美国国家科学基金会;
关键词
Zebra finch; Area X; miR-9; Lentivirus; Song learning; Song performance;
D O I
10.21769/BioProtoc.3006
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Here we provide a detailed step-by-step protocol for using lentivirus to manipulate miRNA expression in Area X of juvenile zebra finches and for analyzing the consequences on song learning and song performance. This protocol has four parts: 1) making the lentiviral construct to overexpress miRNA miR-9; 2) packaging the lentiviral vector; 3) stereotaxic injection of the lentivirus into Area X of juvenile zebra finches; 4) analysis of song learning and song performance in juvenile and adult zebra finches. These methods complement the methods employed in recent works that showed changing FoxP2 gene expression in Area X with lentivirus or adeno-associated virus leads to impairments in song behavior.
引用
收藏
页数:13
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