MONOCLONAL-ANTIBODY ENHANCEMENT OF FSH-INDUCED UTERINE GROWTH IN SNELL DWARF MICE

The enhancing effect of bovine FSH monoclonal antibody (bFSH-MAb) on the gonadotrophic activity of FSH was investigated in dwarf mice using a uterine weight bioassay. Increasing doses of bovine FSH (NIH-FSH-B1; 3.3, 10 or 30 mug/day) were administered for 5 days to dwarf mice (groups of five) with or without administration of a bFSH-MAb preparation (USDA-bFSH-MC28; 100 mug protein/day) which at a dilution of 1:15 000 bound 50% of I-125-labelled bFSH (USDA-bFSH-BP3). The bFSH, at the doses given, gave no increases in uterine. weight; when, however, these doses were given with bFSH-MAb, significant (three- to four fold) increases in uterine weight resulted.