RAPID MTDNA SEQUENCE-ANALYSIS OF FISH POPULATIONS USING THE POLYMERASE CHAIN-REACTION (PCR)

被引：12

作者：

BECKENBACH, AT ^{[1
]}

机构：

[1] SIMON FRASER UNIV,INST MOLEC BIOL & BIOCHEM,BURNABY V5A 1S6,BC,CANADA

来源：

CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES | 1991年 / 48卷

关键词：

D O I：

10.1139/f91-307

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The polymerase chain reaction (PCR) is a relatively new technique allowing the enzymatic amplification of selected genes to produce quantities sufficient for the determination of nucleotide sequences. We have compared sequences of several genes from the mitochondrial genome of six salmonid species. PCR analysis of protein coding genes provides sufficient resolution for determining relationships among species, or among isolated populations. Examination of more variable regions, such as the control region of the mitochondrial genome, will be required for other intraspecific comparisons.

引用

页码：95 / 98

页数：4

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