IDENTIFICATION OF ADPR-TRANSFERASE ACTIVITY IN THE RAT TAPEWORM, HYMENOLEPIS-DIMINUTA

被引：3

作者：

ALSHARIF, M ^{[1
]}

APWEILER, R ^{[1
]}

WHISH, WJD ^{[1
]}

WHITE, CJB ^{[1
]}

BRUNT, RV ^{[1
]}

机构：

[1] BATH COLL HIGHER EDUC, BATH BA2 9BN, ENGLAND

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 96卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0305-0491(90)90051-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. The nuclear fraction of the rat tapeworm Hymenolepis diminuta (Cestoda) contains the enzyme adenosine diphosphoribosyl transferase (ADPR-transferase). 2. 2. The enzyme catalyzes the postsynthetic modification of some nuclear proteins by the covalent attachment of the (ADP-ribose) moiety of NAD to such proteins. 3. 3. The reaction is dependent on DNA which contains strand-breaks, and chain lengths equivalent to (ADP-ribose) is estimated. 4. 4. The formation of polynucleotide products was competitively inhibited by 3-acetamidobezamide, with a Km of 125 μM. 5. 5. The catalytic properties of ADPR-transferase in Hymenolepis diminuta are similar to those in T. brucei. © 1990.

引用

页码：527 / 531

页数：5

共 22 条

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BENJAMIN RG, 1980, J BIOL CHEM, V255, P10943

[2] EFFECT OF 3-AMINOBENZAMIDE ON ANTIGENIC VARIATION OF TRYPANOSOMA-BRUCEI [J].