BIOSYNTHESIS OF PLATELET-ACTIVATING FACTOR (PAF-ACETHER) .2. INVOLVEMENT OF PHOSPHOLIPASE-A2 IN THE FORMATION OF PAF-ACETHER AND LYSO-PAF-ACETHER FROM RABBIT PLATELETS

被引：126
作者：
BENVENISTE, J [1 ]
CHIGNARD, M [1 ]
LECOUEDIC, JP [1 ]
VARGAFTIG, BB [1 ]
机构：
[1] INST PASTEUR, UNITE VENINS, F-75724 PARIS 15, FRANCE
来源：
THROMBOSIS RESEARCH | 1982年 / 25卷 / 05期
关键词：
D O I：
10.1016/0049-3848(82)90128-1
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The role of phospholipase A2 (PLA2) in the formation of platelet-activating factor (PAF-acether) by rabbit platelets is supported by several pieces of evidence. The release of PAF-acether was accompanied by that of lyso-PAF-acether. EDTA, EGTA [ethylene-glycol-bis-(.beta.-aminoethyl ether)N,N''-tetraacetic acid], dibutyryl cAMP, p''-bromophenacylbromide and 874 CB, which, in spite of their structural diversity, are all PLA2 blockers, inhibited the release of both PAF-acether and the lyso-compound. Addition of hog pancreas PLA2 to platelets as well as platelet lysis resulted in the release of lyso-PAF-acether, thus mimicking the metabolic events initiating formation of PAF-acether. PLA2 activation may trigger both the 2nd and the 3rd pathway of platelet activation.
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页码：375 / 385
页数：11
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