Heme oxygenase gene expression in human azoospermic testicular tissue

被引：0

作者：

Abdel Aziz, Mohamed Talaat ^{[1
]}

Shaker, Olfat Gamil ^{[1
]}

Mostafa, Taymour ^{[2
]}

El-Asmar, Farid ^{[3
]}

Atta, Hazem ^{[1
]}

Kamal, Nagwa ^{[1
]}

Mohsen, Gamal ^{[1
]}

Reheem, Mostafa Abdel ^{[2
]}

机构：

[1] Cairo Univ, Kasr El Aini Fac Med, Dept Med Biochem, Mol Biol Unit, Cairo 12311, Egypt

[2] Cairo Univ, Fac Med Kasr El Aini, Dept Androl, Cairo 12311, Egypt

[3] Ein Shams Univ, Fac Med, Dept Med Biochem, Cairo, Egypt

来源：

BIOCHIMICA CLINICA | 2005年 / 29卷 / 5-6期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heme Oxygenase (HO), a microsomal enzyme, catalyzes the rate-limiting step in heme catabolism. To assess the expression of HO-1 and HO-2 genes in human azoospermic testicular tissues, forty seven non-obstructive azoospermic (NOA) men were studied. They were divided according to the results of their testicular sperm extraction (TESE) into; successful positive TESE (n=19) and unsuccessful negative TESE (n=28) compared to 12 obstructive azoospermia cases with matched age. Serum FSH hormone was carried out by ELISA method. Testicular biopsies obtained from azoospermic men were subjected to histopathology, testicular sperm extraction (TESE) and detection of HO-1 and HO-2 gene expression by RT-PCR. The results showed that all azoospermic testicular tissue specimens expressed HO-2 mRNA cases. HO-1 mRNA expression was evident in all obstructive azoospermia and successful TESE positive NOA cases (n=19) as well as in 7/28 unsuccessful TESE negative NOA cases. HO-1 mRNA expression was negative in 21/28 negative TESE cases. It is concluded that HO-1 may play a role in the spermatogenic process.

引用

页码：468 / 474

页数：7

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