POSTRIBOSOMAL COMPLEXES CONTAINING EUKARYOTIC INITIATION FACTOR-EIF-2

被引：1
作者：
AMESZ, H
HAUBRICH, T
VOORMA, HO
机构：
[1] Molecular Biology Section, Department of Molecular Cell Biology, Utrecht, 3584 CH, Transitorium 3
来源：
MOLECULAR BIOLOGY REPORTS | 1979年 / 5卷 / 1-2期
关键词：
D O I：
10.1007/BF00777498
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Eukaryotic initiation factors are found in the postribosomal supernatant as well as bound to the 40S ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with other protein(s) with a sedimentation value of 16-20S. This complexed fraction contains besides eIF-2 another activity which exhibits a profound stimulation on amino acid incorporation in crude lysates and appears to counteract the heme-regulated inhibitor. © 1979 Dr. W. Junk bv Publishers.
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页码：121 / 125
页数：5
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