CLONING AND CHARACTERIZATION OF THE MBOLL RESTRICTION-MODIFICATION SYSTEM

被引：33

作者：

BOCKLAGE, H ^{[1
]}

HEEGER, K ^{[1
]}

MULLERHILL, B ^{[1
]}

机构：

[1] UNIV COLOGNE,INST GENET,WEYERTAL 121,W-5000 COLOGNE 41,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 05期

关键词：

D O I：

10.1093/nar/19.5.1007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The two genes encoding the class IIS restriction-modification system MboII from Moraxella bovis were cloned separately in two compatible plasmids and expressed in E. coli RR1-DELTA-M15. The nucleotide sequences of the MboII endonuclease (R.MboII) and methylase (M.MboII) genes were determined and the putative start codon of R.MboII was confirmed by amino acid sequence analysis. The mboIIR gene specifies a protein of 416 amino acids (MW: 48,617) while the mboIIM gene codes for a putative 260-residue polypeptide (MW: 30,077). Both genes are aligned in the same orientation. The coding region of the methylase gene ends 11 bp upstream of the start codon of the restrictase gene. Comparing the amino acid sequence of M.MboII with sequences of other N6-adenine methyltransferases reveals a significant homology to M.RsrI, M.HinfI and M.DpnA. Furthermore, M.MboII shows homology to the N4-cytosine methyltransferase BamHI.

引用

页码：1007 / 1013

页数：7

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